|
KMID : 0545120100200071140
|
|
Journal of Microbiology and Biotechnology
2010 Volume.20 No. 7 p.1140 ~ p.1151
|
|
|
Characterization of bacterial structures in two-stage moving-bed biofilm reactor (MBBR) during nitrification of the landfill leachate
|
|
Slawomir Ciesielski
Dorota Kulikowska
Ewelina Kaczowka
Przemysław Kowal
|
|
|
Abstract
|
|
|
|
Differences in DNA banding patterns, obtained by ribosomal intergenic spacer analysis (RISA), and nitrification were followed in a moving-bed biofilm reactor (MBBR) receiving municipal landfill leachate. Complete nitrification (> 99%) to nitrate was obtained in the two-stage MBBR system with an ammonium load of 1.09 g N-NH4/m2.d. Increasing the ammonium load to 2.03 g N-NH4/m2.d or more caused a decline in process efficiency to 70-86%. Moreover, at the highest ammonium load (3.76 g N-NH4/m2.d), nitrite was the predominant product of nitrification. Community succession was evident in both compartments in response to changes in ammonium load. Non-metric multidimensional scaling (NMDS) supported by similarity analysis (ANOSIM) showed that microbial biofilm communities differed between compartments. The microbial biofilm was composed mainly of ammonia-oxidizing bacteria (AOB), with Nitrosomonas europeae and N. eutropha being most abundant. These results suggest that high ammonium concentrations select for particular AOB strains.
|
|
|
KEYWORD
|
|
|
AOB, landfill leachate, nitrification, MBBR, RISA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|